Lemuel B. Wingard Jr., Jerome P. Ferrance (auth.), Donald L.'s Biosensors with Fiberoptics PDF

By Lemuel B. Wingard Jr., Jerome P. Ferrance (auth.), Donald L. Wise, Lemuel B. Wingard Jr. (eds.)

Focusing at the speedily expanding interplay among biotechnology and complex fiberoptics/electronics, Biosensors with Fiberoptics emphasizes the 3 significant stages of the developmental technique from suggestion to market: learn, improvement, and applications.

With contributions by way of top specialists without delay concerned about key parts of this fascinating, fast moving box, the e-book describes novel biosensor applied sciences and such present and capability functions as: chemical sensing • fluorescent labels • evanescent-wave biosensors and immunosensors • medical purposes • immunoassay kinetics • luminescence • tracking of environmental toxins.

Researchers and clinicians in a extensive spectrum of disciplines will locate clever and Wingard's Biosensors with FIBEROPTICS an incredibly sensible, updated reference text.

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Using an extrinsic sensor and acid solution, chloride and other halides have been titrated with silver nitrate in the presence of acridine (77). At fIrst, fluorescence is weak by virtue of fluorescence quenching by chloride. As the titration proceeds, the concentration of chloride becomes smaller and reaches a minimum at the end point, when quenching is least efficient. From this point on, the addition of excess silver ion, which acts as a quencher, leads to a decrease in fluorescence. Camara and coworkers (78) have described a fIberoptic sensor to determine sulfIde in aqueous samples.

Eds. (1987) Biosensors: Fundamentals and Application (Oxford University Press, Oxford). 6. Wingard, L. B. Jr. , Hatfield, W. ), Marcel Dekker, New York, in press. 7. Rechnitz, G. A. (1987) Biosensors: Challenges for the 1990s, in Biosensors International Workshop 1987 (Schmid, R. , Guilbault, G. , 24 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. , and Wingard, L. ) VCH, Weinheim, Germany, pp. 3-12. Wingard, L. B. , cds. (1976) Immobilized Enzyme Principles (Academic, New York).

60 ppm by using 1M NaCI as a saline background. The system is quite selective for sulfIde, the most severe interferences being produced by thiosulfate, sulfIte, alkyl mercaptans, and phosphate. The main advantage of Chemical Sensing with Fiberoptic Devices 45 this sensor stems from the stability of the methylene blue immobilized on the solid support, which allows resin to be retained for analysis at a later date. Minimal resin and reagent consumption gives low cost, but the system is irreversible and nonregenerable, thus making this sensor unsuitable for continuous sulfide monitoring.

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