Download e-book for iPad: Fluorescence and Phosphorescence of Proteins and Nucleic by Sergei V. Konev

By Sergei V. Konev

Fluorescence and phosphorescence are proving to be tremendous delicate probes for elucidating conformation of proteins and nucleic acids and for learning molecular interactions. more recent instrumentation and strategies pontificate nice promise for the way forward for those luminescence tools in biopolymer study. It has to be famous, besides the fact that, that the invention that yes amino acids, purines, and pyrimidines emit fluorescence or phosphorescence is comparatively contemporary, taking place in the final decade. Professor Konev is among the pioneers within the software of those approaches to biopolymers and is extremely certified to put in writing approximately this topic. This e-book, notwithstanding written mostly as a monograph of the author's personal contributions, can also be an outstanding evaluation of the topic. Of specific curiosity are the references to many vital Russian papers during this box that have no longer been famous within the Western literature. it truly is obvious from this ebook that fluorescence and phosphorescence tools are getting used approximately as commonly in Russia as in different places on the planet and that we mustn't ever put out of your mind those im­ portant contributions. Konev's stories on protein fluorescence were well known. it really is of curiosity to benefit approximately those and different of his purposes. The final a part of the ebook, which bargains with fluorescence as a method to probe into the constitution and conforma­ tion of macromolecules in intact cells, is finest. apart from released symposia this ebook is the 1st written particularly approximately luminescence of biopolymers. Sidney Udenfriend Bethesda. Maryland could, 1967 v CONTENTS advent . • • . . . . . . . . . • . . . . . . . . . .

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Sample text

Radiation transition) RN- + OH2 - RNH + OH- ELECTRONIC EXCITED STATES OF MONOMERS 27 Following Boaz and Rollefson (1950). who showed the radiationless nature of the R*N- - RN- transition in a-naphthylamine, White regards quenching by hydroxyl ions as a transfer of the H ion from the nitrogen atom of the excited tryptophan molecule to the ion of the OH solvent. White's point of view is shared by Weber (1961). Vladimirov and Li Chin-kuo (1962). and Udenfriend (1962). This scheme. however. requires some amendment.

Scheme of electronic vibrational levels of indole and tryptophan molecules and orientation of corresponding oscillators to the "skeleton" of the molecule. ELECTRONIC EXCITED SfATES OF MONOMERS 53 electronic transition with an absorption maximum at 266 mJL (in hexane) and a maximum at 272 mJL in polar solvents, and to the 1La - A electronic transition inemission(itis entirely responsible for the fluorescence in aqueous solutions), lies in the plane of the molecule at an angle of less than 90° relative to the 1Lb oscillator.

This conclusion is also confirmed by the fact that, when phosphorescence is excited by the 265-mf-L mercury line, the emission polarization spectrum of the phosphorescence of indole is almost a straight line, whereas excitation by the 280-mf-L line produces a spectrum with a distinct structure, which is presumably due to the superposition of two series of electronic vibrational bands polarized in mutually perpendicular directions. The 280-mf-L line, of course, excites a relatively larger proportion of 1Lb oscillators which have a phosphorescence component coinciding in direction with them.

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