Falk, Per's Gut Ecology PDF

By Falk, Per

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Dalwadi H, Kronenberg M, Sutton CL, Braun J. The Crohn’s disease-associated bacterial protein I2 is a novel enteric T cell superantigen. Immun 2001; 15:149–158. 04 17/12/2001 10 26 am Page 25 The ‘unculturables’ Kevin Collins and Liam O’Mahony 4 The flora of the gastrointestinal tract Oesophagus The human gastrointestinal tract is a complex and varied environment for microorganisms. Its beginning, the oesophagus, is a neuromuscular tube with a squamous epithelium. In real terms the oesophagus does not have a colonising commensal flora, but it may have transient bacterial flora from the oral cavity and from dietary sources.

32. Gutgemann I, Fahrer AM, Altman JD, Davis MM, Chien YH. Induction of rapid T cell activation and tolerance by systemic presentation of an orally administered antigen. Immunity 1998; 8:667–673. 25. Briles DE, Nahem M, Schroer K, et al. Antiphosphocholine antibodies found in normal mouse serum are protective against intravenous infection with type 3 33. Helgeland L, Vaage JT, Rolstad B, Midtvedt T, Brandtzaeg P. 05 17/12/2001 10 27 am Page 48 48 Gut ecology in rat intestine. Immunology 1996; 89: 494–501.

Once a specific melting point is reached, dissociation occurs, except at the GC clamp. The open pinlike structure slows dramatically or stops migrating in the gel. This gives rise to a specific band. In practice, bands can be separated based on a single nucleotide difference. Following staining of the gel, a banding pattern can be observed which reflects the diversity of the rRNA genes in the sample. Human adult faecal samples usually yield approximately 20 dominant bands representing, on a semiquantitative basis, the most abundant species within the faecal flora.

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