By Jean-Yves Girard
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Extra resources for Linear Logic
J. (1995) A paradigm for drug discovery employing encoded combinatorial libraries. Proc. Natl. Acad. Sci. USA 92, 6027–6031. 33. , Holmes, C. , Murphy, M. , Jacobs, J. , Gordon, E. , and Gallop, M. A. (1996) Versatile approach to encoding combinatorial organic syntheses using chemically robust secondary amine tags. J. Med. Chem. 39, 1601–1608. 34. Moran, E. , Cargill, J. , Shahbaz, M. , Mjalli, A. M. , and Armstrong, R. W. (1995) Radio frequency tag encoded combinatorial library method for the discovery of tripeptide-substituted cinnamic acid inhibitors of the protein tyrosine phosphatase PTP1B.
Appel, J. , Dooley, C. , and Cuervo, J. H. (1991) Generation and use of synthetic peptide combinatorial libraries for basic research and drug discovery. Nature 354, 84–86. 24. Brenner, S. and Lerner, R. A. (1992) Encoded combinatorial chemistry. Proc. Natl. Acad. Sci. USA 89, 5381–5383. 25. Needels, M. , Jones, D. , Tate, E. , Heinkel, G. , Kochersperger, L. , Dower, W. , Barrett, R. , and Gallop, M. A. (1993) Generation and screening of an oligonucleotide-encoded synthetic peptide library. Proc.
Drain the mixture and wash the resin with 5 mL each of DCM (4×), MeOH (2×), and DCM (4×). 3. Treat the resin in each of the 31 vessels with a unique RC Fmoc-amino acid synthon. 11 mmol). 32 mmol, 3 Eq). Shake the mixture at 25°C. Monitor the coupling reaction in the vessel using ninhydrin test to determine the level of the amine functionality remaining. Upon completion of the coupling reaction (2 h, negative ninhydrin test), drain the mixture and wash the resin with 10 mL each of DMF (3×), MeOH (3×), and DCM (3×).